Aktivitas antioksidan dan efek sitotoksik ekstrak Kola (Cola nitida) pada kulter sel kanker hati (HepG-2)


  • Susi Endrini Department of Biochemestry, Faculty of Medicine YARSI University, Jakarta
  • Himmi Marsiati Department of Biochemestry, Faculty of Medicine YARSI University, Jakarta
  • Suherman J Faculty of Medicine and Health, Muhammadiyah University of Jakarta
  • Fauziah O Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, D.E., Malaysia
  • Asmah R Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, D.E., Malaysia



cytotoxic effects, cola fruit extract, antioxidant, MTT, DPPH


Liver cancer is one among cancers with increasing incidence in the world. Cola fruit (Cola nitida) is a fruit that is rich in properties and has been known since the Dutch colonial era. This fruit contains ingredients such as those contained in tea and chocolate such as methylxanthine and its derivatives. This study aims to determine the content of antioxidants and cytotoxic effects of cola fruit extracts obtained from Indonesia and Malaysia on liver cancer cell lines. Antioxidant content of fruit extracts of cola was assessed using DPPH (2,2-diphenyl-2-picrylhydrazyl hydrate) and cytotoxic effects were studied using MTT (3 - (4,5-Dimethylthiazol-2-Yl)diphenyltetrazolium bromide -2.5) on human liver cancer cell lines (HepG2). The results showed that cola fruit from Malaysia contained high antioxidant with the IC50 value of 37.2 ?g/mL whereas IC50 of value of its Indonesia’s cola fruit was 66.0 ug / mL. The similar results have been shown in the cytotoxic test using HepG-2 liver cancer cell lines. Malaysia’s cola fruit extract has a smaller IC50 value of 6.5 ?g / mL while the fruit extract of Indonesia’s cola showed IC50 value of 39.5 ?g / mL. These values indicates that the fruit extract of cola is a potential anticancer activities especially on liver cancer. Further studies are required to clarify this hypothesis.


Ali AM, Macjen M, Hamid M, Lajis NH, El-Sharkawy S, and Murakoshi M 1996. Antitumor promoting and antitumor activities of the crude extract

from leaves of Juniperus chinensis. J. Ethnopharmacology 53: 165-169.

Australian Naturopathhic Network 2006. Cola nitida. Available on internet at http://www.ann.com.au/herbs/monographs/cola.htm.

Ballard GO 2006. The real truth about diet and weight loss. Available on internet at http://www.titanlabs.com/scweightmanagement.htm

Bertha H 2003. Buah hutan Multikhasiat, TRUBUS, 404 (34): 33 -36.

Brand-Williams W, Cuvelier ME, and Berset C 1995. Use of a Free Radical Method to Evaluate Antioxidant Activity, Lebensmittel-wissenschaft und Technologie, 28, 25-30.

Gurav S, Deshkar N, Gulkari V, Duragkar N, and Patil A 2007. Free Radical Scavenging Activity of Polygala chinensis Linn, harmacologyonline, 2: 245-253.

Maskarinec G 2009. Cancer Protective Properties of Cocoa: A Review of the Epidemiologic Evidence. Nutrition And Cancer, 61 (5): 573-5799.

Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicty assay. J. Immunol.

Methods. 65: 55-63.

Shen DW, Lu YG, hin KV and Gottesman M 1991. Human hepatocellular carcinoma cell lines exhibit multidrug resistance unrelated to MSR1

gene expression. JournaL of Cell Sciences 98:317-322.

Zhong X, Xiong M, Meng X and Gong R 2010. Journal of Experimental and Clinical cancer Research. 29: 115






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